![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4395034:Design
Designed by: Ling Jiajun Group: iGEM22_SYSU-CHINA (2022-10-03)
SpyTag-UGT85A1-SpyCatcher
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 583
Illegal PstI site found at 498 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 583
Illegal PstI site found at 498 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 583
Illegal PstI site found at 498 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 583
Illegal PstI site found at 498 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The idea of fusing SpyTag and SpyCatcher on the N-terminus and C-terminus, respectively, on the enzyme UGT85A1 enables the fusion protein to catalyze glucosyl transfer in higher temperature than one where the reaction normally happens.
Source
The SpyTag/SpyCatcher system is a derivative of CnaB2 domain of Streptococcus pyogenes.UGT85A1 comes from the genome of Arabidopsis thaliana.